We are interested in defining the molecular events that occur during Japanese encephalitis virus (JEV) RNA genome replication. Japanese encephalitis is the most important arbovirus encephalitis in terms of morbidity and mortality in Taiwan and throughout greater Asia. By defining cis- and trans-acting factors involved in the regulation of RNA replication and gene expression, we hope to understand the molecular basis for the switch from acute lytic to persistent nonlytic infection. We have identified two subgenomic RNAs involved in persistent infection of JEV. One is the abundant accumulation of a 3’-terminal 522-nt genome fragment (sfRNA), representing the highly conserved region of the 3’-untranslated region, and the other is defective interfering (DI) RNA in persistently JEV-infected cells. We also identified several host factors involved in viral replication. Understanding of molecular basis of replication mechanism is required for design of therapeutic molecules that block virus replication.
|Program:||「TEEP@AsiaPlus」Mechanism of Viral Replication, JEV, Noncoding RNA|
|School:||National Dong Hwa University|
|Organizer:||Department of Life Science|
|Period of Apply:||
|Tuition Fee:||Please contact us!|
Contact Person: Ms. Laura Li
Website of Program: www.dlife.ndhu.edu.tw/files/11-1026-8029.php